Hypolipidemic effects of N-acetylcysteine in CF-1 mice fed a high-fat diet

MONTALDO LAURA; SANTIAGO ALEJO; MONTALDO LAURA; SANTIAGO ALEJO; FRONTERA EVELYN MICAELA; MARTINI CLAUDIA; FRONTERA EVELYN MICAELA; MARTINI CLAUDIA; GUERRA LILIANA; NAGUILA ZAIRA; GUERRA LILIANA; NAGUILA ZAIRA

Mar del Plata

Congreso; Reunión Anual de Biociencia 2019; 2019

Sociedad Argentina de Investigación Clínica

We showed that that antioxidant N-acetylcysteine (NAC) inhibits cellular lipid accumulation during adipocyte differentiation in vitro (Soto et al, 2016 and Pieralisi et al 2017). Here we evaluated effects of NAC on mice fed a high-fat diet and in vivo lipid parameters. FCEyN ? UBA CICUAL approved this protocol. Thirty-six CF-1 male mice weighting from 39 to 42 g were randomly assigned to 4 treatment groups, fed normal-fat diet with 2,9 Kcal/g without (group C, n= 12 ) or with NAC (group CN, n= 12) and high-fat diet with 5,7 Kcal/g without (group O, n= 6) or with NAC (group ON, n= 6) for 45 days. NAC supplementation in drinking water was 1.2g/L, animals fed and drank ad libitum. We did not observe toxic effect of NAC and all mice consumed similar amount of food and water during treatments. At day 45, we determined mice body weight (BW) and sacrificed them. We evaluated: a) serum cholesterol (Chol) and triglyceride (Tg) levels; b) liver and omental adipose tissue histology; c) omental fat weight (OFW). At day 45 CN had significantly lower BW than C (46.8+-1.2 g [C] vs 42 .0+-0.7 g [CN], p< 0.01); but ON had similar BW as O (43.9+-1.1 g [O] vs 44 .7+-3.1 g [ON], p= 0.5; no significant difference). Nevertheless, O showed significantly higher Chol and Tg levels than C (Chol: 3.32 +-0.05 mmol/L [O] vs 1.92+-0.05 mmol/L [C], p< 0.01; Tg: 2.94+-0.07 mmol/L [O] vs 2.58+-0.11 mmol /L [C], p< 0.01). Chol and Tg levels significantly decreased in ON compared to O (Chol: 2.80 +-0.02 mmol/L [ON], O vs ON p