Effects of fipronil in biomarkers responses in a native species (Cyphocharax voga): branchial ex vivo exposure

SANTILLÁN DEIÚ ANTONELLA; MIGLIORANZA KARINA; LOZANO ISMAEL; DE LA TORRE FERNANDO; ONDARZA PAOLA

Congreso; 12th SETAC Latin America Biennial Meeting; 2017

Fipronil (Fp) is a current use pesticide, highly toxic with potential nocive effects on aquatic organisms. Biomarkers responses are frequently used to detect these effects in fish either on laboratory exposure or in situ assessments. In ex vivo experimental models, the use of gills is justified since it is the first organ in contact with environmental pollutants. Biomarkers responses promoted by two sublethal concentrations of Fp were evaluated in Cyphocharax voga after a branchial ex vivo exposure model. After sacrificing the fish (n=4), branchial arches were dissected and randomly assigned to four treatment groups: saline solution (Ctrl); methanol in saline solution (CtrlSv); and two nominal concentrations of Fp (9 µg/L, C1 and 100 µg/L, C2) prepared from a stock solution on methanol. Gills remained submerged in the solutions under continuous agitation for 1h at 25°C in glass containers. Levels of Fp on water samples were also analyzed with liquid-liquid and GC-ECD extraction. After incubation period, enzymatic activities of superoxide dismutase (SOD), antioxidant capacity against peroxyl radicals (ACAP), lipid peroxidation levels by TBARS and reduced glutathione levels (GSH) were evaluated. Differences between groups were analyzed by one way ANOVA (p