ITA-NOA   24624
INSTITUTO DE TECNOLOGIA AGROINDUSTRIAL DEL NOROESTE ARGENTINO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Molecular characterization of Acidovorax avenae, the causal agent of red stripe, in Tucumán, Argentina
Autor/es:
JOYA, C.; CHAVEZ, S; CASTAGNARO, A.P.; PERERA, M. F.; FUNES, C; CUENYA, M.I.; BERTANI, R; HENRIQUEZ, D; GONZALEZ, V
Lugar:
San Miguel de Tucumán
Reunión:
Congreso; ISSCT XXX Congress; 2019
Resumen:
Red stripe, caused by Acidovorax avenae, has increased in significance in Tucumán, Argentina, over the past decade. The aims of this study were to: i) establish an adequate molecular technique that reflects pathogen genetic diversity of A. avenae isolated from sugarcane producing areas of Tucumán, and ii) correlate genetic diversity with virulence. One hundred and twelve out of the 321 A. avenae suspected samples were confirmed by species-specific PCR. A. avenae isolates were characterized by repetitive element polymorphism-based polymerase chain reaction (rep-PCR) and Amplified Fragment Length Polymorphism (AFLP), and a high level of genetic diversity was found. However, there was no apparent correlation between the isolate groups and sugarcane host genotype, its age or the type of tissue sampled, fertilization, or the year of sampling. Six A. avenae isolates (belonging to different rep-PCR similarity groups) were evaluated for pathogenicity and no statistically significant correlation between the virulence of the isolates and the genetic group that they belong to was found. The better understanding of the genetic and pathogenic diversity obtained and the existence of genotype-genotype specific interactions between sugarcane varieties and A. avenae isolates could allow developing management strategies.