Temporary immersion system as an alternative method for the micropropagation of a recalcitrant sugarcane genotype

DÍAZ, ME; CASTAGNARO, AP; NOGUERA, AS; INSAURRALDE ROCCO, P; PAZ, N; PERERA, M. F.

San Miguel de Tucumán

Congreso; ISSCT XXX Congress; 2019

Although micropropagation is the most utilized tissue-culture technique to scale up plant production, it has a low multiplication coefficient and little possibility of process automation. In this sense, Temporal Immersion Bioreactors (TIB) could overcome those disadvantages. The aim of this work was to optimize an alternative micropropagation technique that allows the production of large quantities of sugarcane genotypes that otherwise are recalcitrant in conventional micropropagation systems. Plantlets of TUC03-12 genotype in the fourth sub-culture step were tested under both production systems (TIB and conventional). Five plantlets were placed in each culture flask to propagate through the conventional method. For the TIB system, 5-L disposable water bottles were used with 25 plantlets per bottle. For TIB, cycles of 5 minutes of immersion every 3 h were performed. The culture chamber was maintained at 25  2C with light periods of 16 h and 2000 lux. After 40 days the efficiency of the two propagation systems were evaluated considering the multiplication coefficient. For the conventional system the multiplication coefficient was 1:7, while in the TIB system it was 1:30. The use of TIB system allowed a substantial increment in the multiplication coefficient of TUC03-12 and stimulated more vigorous and better-quality plants than the conventional micropropagation system.