ITA-NOA   24624
INSTITUTO DE TECNOLOGIA AGROINDUSTRIAL DEL NOROESTE ARGENTINO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Asessment of inoculation techniques for red stripe disease resistance screening
Autor/es:
HENRIQUEZ, D.; BERTANI, R; PERERA, M. F.; JOYA, C.; CUENYA, M. I.
Lugar:
San Miguel de Tucumán
Reunión:
Congreso; ISSCT XXX Congress; 2019
Resumen:
Red stripe (Acidovorax avenae) is one of the main bacterial diseases that affects sugarcane in Tucumán, Argentina. This disease has become important in our country due to its high incidence over the past 10 years. Host-plant resistance is the most effective method for disease control and breeding programs require effective screening techniques to select sugarcane genotypes resistant to A. avenae. The aim of this study was to optimize a practical method that simulates natural disease spread and allows reproducible expression of red stripe symptoms. Three inoculation techniques (spraying the bacterial suspension after rubbing the leaf surfaces using refined sand or cotton, and spraying without making a wound) were tested under controlled conditions. Relative humidity, soil composition and fertilization were optimized to maximize symptom expression. All methods tested reproduced typical disease symptoms; however, the mechanical inoculation based on rubbing both leaf surfaces with refined sand was the most aggressive. The no-abrasion method simulates natural infection conditions, where the dispersion of A. avenae is by wind and rain and is the method that is the most effective in a breeding program for disease-resistance screening. These results represent a valuable contribution for sugarcane breeding programs that evaluates red stripe resistance of their material.