ITA-NOA   24624
INSTITUTO DE TECNOLOGIA AGROINDUSTRIAL DEL NOROESTE ARGENTINO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
PRODUCTION OF PHENOLIC COMPOUNDS FROM IN VITRO SHOOTS OF SUGARCANE (SACCHARUM SPP.) IN TEMPORARY IMMERSION BIOREACTORS
Autor/es:
ACEVEDO ROJAS, R; BERNAL VILLEGAS, A.; NOGUERA, A. S.; CASTILLO, R.; DANIELS, D.; GRELLET BOURNONVILLE, C. F.; LA O HECHEVERRIA, M.; GOMEZ COSLY, R.; CASTAGNARO, A. P.
Lugar:
Yerba Buena
Reunión:
Congreso; International Society of Sugar Cane Technologists (ISSCT) 2019; 2019
Institución organizadora:
International Society of Sugar Cane Technologists (ISSCT)
Resumen:
An alternative way to obtain molecules of high-value for the agricultural and pharmaceutical industries is via the production of secondary metabolites from in vitro systems. The objective of this work was to establish the culture conditions for the production and scaling up of phenolic compounds from sugarcane shoots produced in Temporary Immersion Bioreactors (TIBs). Different sucrose concentrations and inoculum densities of shoots per flask were tested for two sugarcane cultivars C86-56 and C1015-73. These cultivars were micropropagated in TIBs without ascorbic acid and the phenolic compound production were measured during 25 days. The best established conditions were evaluated on a pilot scale with ten TIBs for three sugarcane cultivars (C86-56, C1051-73 and C87-51). The most important production was achieved by using 15 shoots per vessel with 20 g.L-1 of sucrose in the absence of ascorbic acid. Cultivar C1051-73 had a higher multiplication coefficient independently of the treatments; this effect was not only significant for the phenolic compound production, but also for biomass increases. In the pilot experiments, the phenolics production was different among three cultivars with C1051-73 showing the highest yield. The results revealed that TIBs are a useful method to produce phenolic compounds with optimum yields.