ITA-NOA   24624
INSTITUTO DE TECNOLOGIA AGROINDUSTRIAL DEL NOROESTE ARGENTINO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Transcriptome profiling of canker resistant Bs2-transgenic citrus plant revealed the up-regulation of disease response genes.
Autor/es:
SENDÍN, LORENA NOELIA; MARANO, MARÍA ROSA; FILIPPONE, MARÍA PAULA; GÓMEZ, ROCIO LILIANA; YU,QIBIN; DU, DONGLIANG; GMITTER, FREDERICK ; CASTAGNARO, ATILIO PEDRO
Lugar:
Montevideo
Reunión:
Simposio; IV INTERNATIONAL SYMPOSIUM ON CITRUS BIOTECHNOLOGY.; 2018
Institución organizadora:
INIA Las Brujas Experimental Station
Resumen:
Abstract body text: Pepper Bs2 gene confers resistance to Xanthomonas campestris pv. vesicatoria (Xcv) pathogenic strains containing the avrBs2 avirulence gene in susceptible pepper and tomato varieties. The avrBs2 gene is highly conserved among members of the Xanthomonas, and avrBs2 of Xcv shares 96% homology with the avrBs2 of Xanthomonas citri subsp. citri (Xcc), causal agent of citrus canker disease. In our previous work, transgenic plants of Citrus sinensis cv. Pineapple expressing Bs2 under the control of a pathogen-inducible promoter (glutathione S-transferase from potato) were obtained. In Bs2-lines, reduction of canker disease symptoms was observed and was dependent on the presence of the Xcc avrBs2 gene. In order to know the molecular mechanisms underlying the differences between Bs2-transgenic and non-transgenic plants after infection with Xcc, transcriptomic profiles of both plants were compared by using RNAseq. A total of 2022 genes were differentially expressed (DEG) in transgenic plant compared with non-transgenic plants, among them, 1356 genes were up-expressed and 666 were down-expressed. Gene ontology (GO) annotation and Singular Enrichment Analysis (SEA) of the DEGs showed that genes related to the biotic stress were the most highly induced in Bs2-transgenic (36.7%), some of these were β-1,3-glucanase, PR4 and PR5 proteins. Moreover, several genes codifying receptors like kinases were also induced (15.6%), followed by transcription factors like WRKY, MYB and bHLH family (9.5%). These results show that Bs2 gene from pepper triggers molecular mechanisms involved in defence response in citrus plants Xcc-infected and can be used to confer resistance in other crops of agronomic importance where Xanthomonas species are pathogens. Moreover, the identification of these genes could be an important step towards developing different strategies to improve citrus response not only to Xcc but also to other diseases affecting this crop, like Huanglongbing.