ITA-NOA   24624
INSTITUTO DE TECNOLOGIA AGROINDUSTRIAL DEL NOROESTE ARGENTINO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Development of a plant defense biostimulant for sustainable crop disease management based on the fungal elicitor AsES
Autor/es:
WELIN BJORN; MORETTI ENRIQUE R. ; DURMAN SANDRA B. ; CHALFOUN NADIA R.; CASTAGNARO ATILIO P.; STENGLEIN SEBASTIÁN A.
Lugar:
Varadero
Reunión:
Congreso; Biotecnología Habana 2017: La Biotecnología Agropecuaria en el Siglo XXI; 2017
Institución organizadora:
Centro de Ingeniería Genética y Biotecnología (CIGB)
Resumen:
In this work we present the first member of the Plant Stimulation and Protection Technology (PSPT), a new line of bioproducts for sustainable crop production and disease management. The first product, PSP1, is based on the elicitor subtilisin AsES, secreted by the strawberry fungal pathogen Acremonium strictum. In an initial step, fungal fermentation conditions were determined to maximize extracellular protein production and to obtain a high plant defense-eliciting activity, as monitored by reduced anthracnose development in supernatant-treated strawberry plants. Disease protection studies using dilutions of PSP1 indicated that there is a threshold activity/concentration of AsES needed to induce pathogen defense in strawberry and that this induction seems to be dose-independent.An important characteristic of PSP1 is its capability to activate disease protection in several different crop species against various pathogenic microorganisms. In more detail, foliar application of PSP1 on soybean generated superoxide anion accumulation, up-regulated PR1-gene expression and protection against infection of Corynespora cassiicola, causal agent of soybean target spot. Furthermore, a double PSP1 treatment on greenhouse-grown sugarcane plants provided protection against bacterial red stripe disease caused by Acidovorax avenae. Likewise, a double foliar application of PSP1 on field-grown wheat plants significantly reduced symptoms of Fusarium head blight disease, which was manifested in higher yields and an increased germination percentage of fungus-infected seeds collected from treated plants.In summary, these disease protection results demonstrated against both bacterial and fungal pathogens in monocot and dicot crop species, together with the PSP1 characteristics of being straightforward and economical to produce, effective at low concentrations, stable and long shelf-life, tolerant to exposure to high temperatures, harmless to non-target organisms, easy to handle and apply and gives excellent protection against pathogen attacks, make PSP1 a very promising ecological candidate for effective and sustainable crop disease management.