ITA-NOA   24624
INSTITUTO DE TECNOLOGIA AGROINDUSTRIAL DEL NOROESTE ARGENTINO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
PEPPER BS2 GENE IS FUNCTIONAL IN CITRUS AND INCREASES CANKER RESISTANCE
Autor/es:
ORCE, I.G.; RUBIO, A.C.; FILIPPONE M.P,; GÓMEZ, R. L.; ATHAYDE, M.G. ; SENDÍN, L.N.; ROMERO, A.L.; CASTAGNARO, A.P.
Reunión:
Congreso; International Citrus Congress, Fos do Iguazu; 2016
Resumen:
Pepper Bs2 gene confers resistance to Xanthomonas campestris pv. vesicatoria (Xcv) pathogenic strains containing the avrBs2 avirulence gene in susceptible pepper and tomato varieties. The avrBs2 gene is highly conserved among members of the Xanthomonas and avrBs2 of Xcv shares 96% homology with the avrBs2 of Xanthomonas citri subsp. citri (Xcc), causal agent of citrus canker disease. In this work, the effect of stable expression of Bs2 gene on citrus canker resistance was evaluated in transgenic plants of Citrus sinensis (L.) Osb. cv. Pineapple. Agrobacterium-mediated transformation was unsuccessful when a constitutive promoter (35SCaMV) was used in the plasmid construction, but transgenic lines were obtained with a construction harbouring Bs2 under the control of a pathogen-inducible promoter, the glutathione S-transferase gene from potato. In Bs2-lines, reduction of canker disease symptoms was observed and was directly depending on the Xcc avrBs2 gene. Additionally, this reduction was correlated with levels of Bs2 gene expression in transgenic plants, as assessed by real-time qPCR and accompanied by production of reactive oxygen species. These results indicate that pepper Bs2 resistance gene is also functional in other different family to Solanaceae and could be considered for canker control. Financial support: EEAOC - CONICET. Keywords: citrus breeding; agrobacterium-mediated; transformation.