ITA-NOA   24624
INSTITUTO DE TECNOLOGIA AGROINDUSTRIAL DEL NOROESTE ARGENTINO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Morphological and molecular characterization of Puccinia kuehnii Cuban isolates.
Autor/es:
BERTANI, R.P.; PERERA, M.F.; ARIAS, M.E.; LAO HECHAVARRÍA, M.; ZARDÓN NAVARRO, M.A.; DEBES, M.A.; LUQUE, A.C.; ALONSO TERRY, E.I.; RODRIGUEZ LEMA, E.I.; CUENYA, M.I.; ACEVEDO ROJAS, R.; PLOPER, L.D.; CASTAGNARO, A.P.
Reunión:
Workshop; ISSCT (International Society of Sugar Cane Technologist) XI Pathology and IX Entomology Workshops; 2015
Resumen:
Orange rust (Puccinia kuehnii) is a widespread sugarcane disease, known to have devastating impacts on the crop. Although the most effective way to control rust diseases is the use of resistant varieties, durability of resistance is conditioned by several factors including pathogen genetics. In Cuba, where orange rust is a relatively new disease and environmental conditions favor infection, there have not been previous studies characterizing the pathogen. The aims of this study were to establish morphological characters that could best describe and differentiate P. kuehnii isolates collected throughout different growing seasons and locations in Cuba, and to set an adequate molecular technique for estimating P. kuehnii genetic diversity. All samples showed typical lesions by P. kuehnii: pustules were located on the abaxial epidermis of leaves and, to a lesser degree, on their adaxial surface in some of the analyzed sugarcane genotypes. In addition, new morphological features associated with this pathogen were observed. Morphological characterization suggested that there is a single, but highly diverse and complex pathogen population, and the groups obtained showed no apparent correlation among isolates and sugarcane varieties, locations and collection dates. With regard to molecular studies, AFLP markers successfully characterized orange rust isolates and detected a higher genetic diversity than morphological descriptors, showing a weak correlation between the isolates and their geographical origins, as well as with the sampling dates. In addition, transferability of Puccinia melanocephala microsatellite loci could not be observed for P. kuehnii. On the other hand, the sequences of rDNA regions did not show significant differences among Cuban P. kuehnii isolates. This work presents a thorough characterization of P. kuehnii isolates, including morphological traits, molecular markers and rDNA region sequence