Heat shock triggers MAPK activation and MKP-1 induction in Leydig testicular cells.

GOROSTIZAGA ALEJANDRA; BRION LAURA; MALOBERTI PAULA; MACIEL FABIANA CORNEJO; PODESTÁ ERNESTO JORGE; PAZ CRISTINA

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Academic Press

Año: 2005 vol. 327 p. 23 - 28

0006-291X

Testicular function is highly dependent on temperature control. In Leydig testicular cells, the signaling pathway activated by heat stress is poorly defined. Here we describe the molecular events triggered by heat shock (HS, 10 min, 45 degrees C) in MA-10 cells. HS produced a rapid and transient activation of ERK1/2 and JNK kinases, and also increased MAP kinase phosphatase-1 (MKP-1) protein and mRNA levels. The effect of HS on MKP-1 messenger reached significance at 15 min, peaked (3.5-fold) at 60 min, and was partially dependent on ERK1/2 activity. The temporal profiles of MKP-1 protein levels and MAPKs phospho-dephosphorylation suggest that MKP-1 induction could contribute to ERK1/2 and JNK inactivation after HS. In summary, this study indicates that the response to heat stress in Leydig cells includes the activation of MAPKs related to cell survival (ERK1/2) and death (JNK), and the induction of a MAPK activity inhibitory loop.