Design and Preliminary Characterization of a fusion protein with NADPH Peroxidase Activity

SKAFAR V; PORTILLO-LEDESMA S ; VAZQUEZ D; DEGENHARDT MFS; OLIVEIRA CLP; SANTOS J.; NETTO LES; FERRER-SUETA G

Congreso; Gordon Research Conference: Thiol-Based Redox Regulation and Signaling; 2018

GRC

We designed a fusion protein containing the sequences of thioredoxin reductase (TR), thioredoxin (Trx) and thiol peroxidase (TPx), all from Escherichia coli. Including the Gly and Thr-containing connecting peptides, the sequence of the construction Trx-TG20T-TR-GTG8T-TPx