The Carbonic Anhydrase Domain of The Carbonic Anhydrase Domain of mitochondrial respiratory Complex I mitochondrial respiratory Complex I is essential to sustain embryogenesis is essential to sustain embryogenesis in in Arabidopsis thaliana

CÓRDOBA, JUAN PABLO; SOTO, DÉBORA; MARTIN, MARÍA VICTORIA; PAGNUSSAT, G

Mar del Plata

Congreso; X V C L A F V - X X X R A F V - Mar del Plata 2014; 2014

Sociedad Argentina de Fisiología Vegetal

The Arabidopsis NADH  dehydrogenase complex shows  some special  subunits such as  the Gamma-Carbonic Anhydrases (γCAs).  The Arabidopsis genome contains five genes  named CA1 (At1g19580), CA2 (At1g47260), CA3  (At5g66510), CAL1 (At5g63510) and CAL2  (At3g48680). The encoded proteins are imported to mitochondria and forms the Carbonic Anhydrase domain (CA domain).  Thisdomain is attached to the membrane armof the Complex I, facing to the mitochondrial matrix. In plants, it was linked to photorespiration.On the other hand,  embryogenesis involves the generation of the basic body  plan  and the establishment of the apical-basal  axis of the mature plant,  with the production of all plant structureprimordia. The stages of embryogenesis have been defined according to the number of cells derived fromthe apical cell or the shapes of the developing embryo (see figure 1).In this work, an Arabidopsis embryo lethal mutant was analyzed. These results show that CA domain is important for respiration and consequently essential to sustain embryogenesis.