Cold-Induced Expression of an Alkaline/Neutral Invertase Isoform in Two Contrasting Wheat Cultivars

LECHNER, LEANDRA; MARTIN, MARÍA VICTORIA; SALERNO, GRACIELA

Portland

Congreso; Plant Biology 2014; 2014

American Society of Plant Biologists

Alkaline/Neutral Invertases (A/N-Inv) are sucrose hydrolyzing enzymes with pH optima in the range of 6.8-8.0, which have been identified in plants and cyanobacteria but their physiological function is still not fully elucidated. In plants, they have cytosolic or organelar location and were suggested to regulate the entry of sucrose into different cytosolic pathways. Also it was reported that they are involved in stress responses in plants. In this study, we determined the expression pattern of an N/A-Inv isoform in different organs of wheat seedlings of two cultivars (Baguette and BioInta, winter and spring cultivars, respectively), in response to cold stress. Differential expression patterns (at the mRNA level) were obtained in root and leaf tissues, and between cultivars. While the growth of primary roots significantly decreased in both cultivars under the cold treatment (4°C), the length of the first emerged leaf of the winter cultivar was not affected by the environmental condition. Interestingly, A/N-Inv transcript levels were higher in Baguette than in BioInta tissues. Our results support the role of A/N-Invs in relation to abiotic stresses and that may be involved in responses to cope with them. Supported by PIP 134, UNMdP (EXA645), PICT 1288, and FIBA.