Radical Scavengind Activity of Argentinean Peanut Skins Extracts (Arachis Hypogaea) in Relation to trans-resveratrol Content

VALERIA NEPOTE; N R GROSSO; C A GUZMAN

Anales de la Asociación Química Argentina

Asociación Química Argentina (AQA)

Año: 2004 vol. 92 p. 41 - 49

0365-0375

The objective of this work was to determine radical scavenging activity in relation to the content of total phenolic compounds and trans-resveratrol (resveratrol) in fractions from ethanolic extracts of peanut skins from Argentina. Ethanolic extract was prepared from defatted peanut skins. Total dry matter content, total phenolic content and radical scavenging activity percentage were determined from ethanolic extract and purified fractions. The ethanolic extract was separated by partition with dichloromethane, ethyl acetate and water. The ethyl acetate fraction was separated in a minicolumn packed with Sephadex LH-20 because this fraction showed higher content in phenolic compounds and radical scavenging activity. The fractions from  the column were analyzed by paper chromatography and thin layer chromatography to identify resveratrol. HPLC and 1H-NMR spectroscopy were performed in resveratrol fraction. Ethanol extracted 0.1056g of dry matter per gram of defatted peanut skins. Ethanolic extract showed 0.932g/g of total phenolic compound and 31.5% of radical scavenging activity. The resveratrol content was 91.4 ± 7.3ug/g in ethanolic extract and 9.07 ± 0.72ug/g in peanut skins.