Role of Acyl-CoA synthetase 4 in mitochondrial bioenergetics in breast cancer cells

DATTILO M; MORI SEQUEIROS GARCÍA MM; MALOBERTI PM; HELFENBERGER KE; CASTILLO AF; BENZO Y; RINGELMANN SANCHEZ P; PODEROSO C

Mar del Plata

Congreso; LXIV Annual Meeting of Argentine Society of Clinical Investigation; 2019

Sociedad Argentina de Investigaciones Clínicas

Acyl-CoA synthetase 4 (ACSL4) catalyzes acyl-CoA synthesis from long chain fatty acid, being arachidonic acid its preferred substrate. In breast cancer, ACSL4 promotes tumor aggressiveness by increasing migration, proliferation and invasion. In cancer cells, there is evidence of dysregulation of mitochondrial function, mitochondrial mass and subcellular spatial organization. The aim of this work is to study whether mitochondrial metabolism is regulated by ACSL4 in breast cancer cells. We evaluated levels of specific proteins in mitochondrial fraction of the MCF-7 stable breast cancer cell line overexpressing ACSL4 (MCF-7 tet-off/ACSL4) and control cells (MCF-7 tet-off/empty vector). We observed a significant increase in Complex III (p