Characterizaton of L-leucine transport in S.cerevisiae grown in medium supplemented with M.oleifera

PICHETTO OLANDA I, CONGOST C, GRONDONA G, MÓNACO C, CHAPELA SP, BURGOS HI, ALONSO M. AND STELLA C.A.

Entre Rios

Congreso; LIV Reunión Anual de SAIB; 2018

YeastYeast YeastYeastmodel systemsystemsystemsystemsystem is suitable suitable suitable to studystudy studythe effecteffecteffecteffect effectof different differentdifferentdifferentdifferentdifferent differentsubstances substancessubstancessubstances substances substanceson the metabolism metabolismmetabolism metabolism metabolismmetabolismof eukaryotic eukaryoticeukaryotic eukaryoticeukaryotic cells cells .TheThe systemsystemsystemsystemsystem avoidsavoidsavoids avoidseffectseffectseffectseffects effects present present presentin cells cells of higher higher higher eukaryotes eukaryoteseukaryotes eukaryoteseukaryotes eukaryotes due dueto organ organorgan or tissues tissuestissues interaction interactioninteraction interaction interaction .TeaTea extractsextracts extracts extracts of M.oleifera oleifera oleifera oleifera(?Moringa (?Moringa (?Moringa ?) have havehavebeen been consideredconsidered considered consideredconsidered beneficial beneficial beneficial beneficial beneficialto be incorporated incorporatedincorporated incorporatedincorporated incorporatedincorporatedincorporated into intointothe diet dietdietof diabeticdiabetic diabeticdiabeticdiabetic patients patients patients or with antioxidant antioxidant antioxidant antioxidant antioxidantantioxidantproperties properties properties properties .However, However,However,However, However,these these beneficial beneficial beneficial beneficial beneficialeffectseffectseffectseffects effectseffectshave havehavenot notbeen been elucidated elucidated elucidated elucidatedelucidatedelucidated from from the point point pointof view of cellular cellular cellular cellularbiochemistry biochemistry biochemistry biochemistry biochemistry biochemistry.TheThe objective objective objective objectiveof the presentpresentpresent presentworkwork workis to characterize characterize characterize characterizecharacterize characterize characterizethe thetransport transport transport transportof the amino aminoacid acid L-leucine leucine leucine leucinein acultureculturecultureculture medium medium supplemented supplemented supplemented supplementedsupplemented with M.oleifera oleifera oleifera oleifera.Extracts Extracts Extracts Extracts of C.sinensis sinensis (?green?) (?green?) (?green?) (?green?) andandandC.angustifolia angustifolia angustifoliaangustifolia angustifolia angustifolia(? sen ?) werewere wereused forfor comparisoncomparison comparison comparison comparison .ResultsResults Resultsobtained obtained obtained obtainedshow that thatthethe transport transport transport of the aminoaminoamino aminoacid acid is increased increased increased increased in all allmedia media with with respectrespect respect to thethe controlcontrol control control medium medium with yeastyeast yeastyeastextract/peptoneextract/peptone extract/peptone extract/peptone extract/peptone extract/peptoneextract/peptone extract/peptone(YPD) (YPD) .TheThe generalgeneral general general amino aminoacid acid systemsystemsystemsystemsystem (GAP (GAP1)wouldwould would be responsibleresponsible responsible responsible forfor the observed observed observedobserved phenotype phenotype phenotype .In the casecase of the sen extractextractextract extractextractextractit is foundfound found that thatit produces produces produces produces aphenotype phenotype phenotype highly highly highly sensitive sensitive sensitiveto the presence presence presence of H2O2in the solid culture culture culturethat cancan be reversedreversedreversedreversed reversed by the theaddition addition addition of antioxidant antioxidant antioxidant antioxidant antioxidantantioxidantN-acetyl acetyl cysteine cysteinecysteinecysteine cysteine .Both Both sen and green green greenteatea increase increase increase increase the thedegradation degradation degradation degradationdegradation of H2O2which whichcancan be reasonedreasoned reasoned reasonedas to agreatergreatergreater greatergreater activity activity of the catalasecatalasecatalasecatalase catalase enzyme enzymeenzyme .ResultsResults Results cancan be interpreted interpretedinterpreted interpreted interpretedinterpretedinterpreted consideringconsidering considering considering consideringconsideringthat thatthe extractsextracts extracts extracts provide provide provide apoor poor source sourcesource of nitrogen nitrogen nitrogen nitrogen analogous analogous analogous analogous analogousto the aminoamino aminoacid acid L-proline proline proline .ThisThis Thisaminoamino acid acid requiresrequires requires requiresrequires greater greatergreatergreatergreater activity activity of mitochondrial mitochondrial mitochondrial mitochondrial mitochondrialoxidativeoxidativeoxidative oxidativeoxidative oxidativemetabolism metabolismmetabolism metabolism metabolismmetabolismand concomitantly concomitantly concomitantly concomitantly concomitantly there therethereis an increase increase increase increase in nonspecific nonspecific nonspecific amino acids acids acidstransport transport transport systemssystemssystemssystemssystems activity activity .