ACYL-COA SYNTHETASE 4 LEVELS ARE DEPENDENT ON PROTEASOME ACTIVITY AND MODULATE MITOCHONDRIAL METABOLISM REGULATORY PROTEINS EXPRESSION IN BREAST CANCER CELLS.

KATIA HELFENBERGER; PAULA MALOBERTI; MELINA DATTILO; CECILIA PODEROSO; YANINA BENZO; LUCÍA HERRERA

Buenos Aires

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS, 2017; 2017

Sociedades Argentinas de Biociencias

Acyl-CoA synthetase 4 (ACSL4) is an enzyme that catalyzes acyl-CoA synthesis from long chain fatty acid, being arachidonic acidits preferred substrate. ACSL4 is overexpressed in triple negativebreast cancer cells correlating with tumor aggressiveness. Wedemonstrated that ACSL4 expression is regulated by transcriptionalmechanisms in breast cancer cells. But other mechanisms involvedin regulating ACSL4 levels might be taken place. It is known thatin cancers exists a strong mitochondrial metabolism deregulation.Computational data showed that ACSL4 is a possible candidate formodulating mitochondrial master regulatory genes. Then, our goal isto study stability of ACSL4 by post translational mechanisms and tostudy the role of ACSL4 in the regulation of mitochondrial function.ACSL4 stability was tested on MDA-MB-231 breast cancer cell linewith cycloheximide (CHX) treatment. Immunoblot showed a time-dependentdecrease in ACSL4 levels after CHX treatment, significantat 4h of CHX (without vs. with CHX 4h: 1 vs 0.5, relativized to control*p