Parenteral succinate reduces reactive oxygen species production in septic rats

CHAPELA S.P., BURGOS H.I., CANZONIERI R., BALLESTERO F.M., BLASCO M.A., MURYAN A., ALONSO M. AND STELLA C.A.

Congreso; XIII Intensive Medicine World Congress and XXII Brazilian Congress of Intensive Care Medicine (CBMI).; 2017

Objective: To observe if administration of succinate reducesReactive Oxygen Species (ROS) production and creatinine levels.selection more accurately than the traditional approach. MOPOCUShelped to perform and/or cancel procedures whichin some patients were lifesaving.Methods: Sprague Dawley rats were used. 4 groups wereformed, with 5 rats each. Group1: control. Group2:5mmol/kg of intraperitoneal (IP) succinate solutionwere administered. Group3: The caecum was ligated andpunctured and 20 ml/kg of intraperitoneal 0.9% NaCland antibiotics were administered. Group4: Cecal Ligationand punction as group3 with 5mmol/kg of IP succinatesolution, and 5mmol/kg of IP succinate solution wereadministered 2 hours before taking the sample. ROS levelswere measured using the Dichlorofluorescein-Diacetate(DCFH) technique.Results: Average DCFH emissions: group1 = 0.0332(SD=0.008), group2 = 0.0352 (SD=0.011), group3 =0.0759 (SD=0.037) and group4 = 0.0598 (SD=0.006).There was significant differences in the 4 groups (p=0.016),and no differences between groups 1 and 2 (p=0.3), and 1and 4 (p=0.3), with significant differences between 1 and3 (p=0.007) and 3 and 4 (p=0.007). Creatinine values(mg/dl): group1 = 0.396 (SD=0.0713), group2 = 0.400(SD=0.0707), group3 = 0.54 (SD=0.0894) and group4 =0.6 (SD=0.2). There was a significant difference among the4 groups (p=0.0353). no correlation between DCFH andcreatinine levels (rho=0.24; p=0.3).Conclusion: Administration of succinate reduced ROSproduction in septic rats, with no decrease in creatininelevels. There isn´t correlation between ROS and creatinine