Soluble guanylyl cyclase alpha1 subunit as a potential biomarker to evaluate estrogen-like effects of endocrine disruptors

RONCHETTI SA; RICCI AG; CORDEIRO G; CABILLA JP; GURRUCHAGA A; DUVILANSKI BH

Mar del Plata

Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2016

SAIC

Endocrine disruptors (EDs) are compounds that interfere in the action of endogenous hormones. A particular class of EDs called xenoestrogens (XEs), mimic cell responses normally induced by estrogen (E2). The main nitric oxide receptor soluble guanylyl cyclase (sGC) is a cytosolic heterodimer composed by two subunits, alpha and beta, and catalyzes cGMP formation. It is ubiquitously present throughout all the zoological scale. E2 differentially affects sGC subunits by increasing alpha1 (a1 and decreasing beta1 (b1) expression. Previously we have shown that a1 expression is particularly sensitive to E2 levels in vivo and in vitro. The aim of the present work was to investigate the expression of a1 as an indicator of EDs exposition in some estrogen-responsive cell lines. Lactosomatotroph-derived pituitary cell line GH3 and endometrial tumor cell line ECC-1 were incubated with several XEs which binds estrogen receptor for 48 h. a1 and b1 expression was determined by western blot. 1 nM cadmium (Cd) and 1 nM arsenic (As) treatments increased a1 expression in GH3 cells (a1 relative units (RU) as % of control; Cd: 152±10*; As: 145.6±13*, E2: 236.5±21.2***, *p