EFFECT OF N-ACETYLCYSTEINE AND IDEBENONE DURING ANAEROBIC INCUBATION OF Saccharomyces cerevisiae

SEBASTIÁN PABLO CHAPELA; MILAGROS P. MANCINI; HILDA ISABEL BURGOS; MANUEL ALONSO; CARLOS A. STELLA

Rosario

Congreso; 50 Reunión de la Sociedad Argentina de Investigación Bioquímia y Biología Molecular; 2014

Sociedad Argentina de investigación Bioquímia y Biología Molecular - SAIB

There are circumstances in which cells are subjected to the absence or reduction of oxygen level. Re-oxygenation may exceed the capacity of the cell to metabolize the received oxygen. Thus ROS increase can lead to diverse cellular alterations. We used yeast S.cerevisiae as a model system to study cellular processes involving mitochondrial metabolism since its ability to grow under aerobic and anaerobic conditions. The aim of this study was to: a) assess cell viability under absence of oxygen, b) relate viability observed with the ROS formation and mitochondrial membrane potential and c) to study N-acetylcysteine (NAC) or idebenone effect in cycles of absence/presence of oxygen. Cells were incubated at 30 °C using an anaerobic jar and a Oxoid generation system. Incubation under aerobic and anaerobic conditions, was performed in the presence of NAC or idebenone. We measure ROS using diclhorofluorescein 2,7-diacetate signal and mitochondrial membrane potential with rhodamine 123. NAC reduces mitochondrial membrane potential in anaerobiosis and ROS production in aerobiosis. Idebenone, increases only the membrane potential in anaerobiosis. Results shows that NAC would be more advantageous as an antioxidant protector under both conditions. Instead, idebenone may be appropriate to enhance the functioning of mitochondria under low oxygen supply or deficient functioning of the organelle.