StAR

CASTILLO, A.F.; PODESTÁ, E.J.; PODEROSO, C.; MALOBERTI, P.M.; MELE P.

Encyclopedia of Signaling Molecules

Springer, New York. Plataforma: Meteor

Lugar: New York; Año: 2017; p. 1 - 10

For many years now, several studies in steroid-producing systems have led to the postulation of an induced stimulatory protein originally designated as the labile protein factor since after stimulation with ACTH in the presence of a protein synthesis inhibitor as cycloheximide (CHX), the rate of steroidogenesis rapidly returned to its unstimulated level (Haynes et al. 1959; Garren et al. 1965; Schulster et al. 1970). Using two-dimension gel electrophoresis, one of the first papers in the matter identified a protein of 28,000 Da, present as different isoforms in hormone-stimulated adrenal cells but not in quiescent cells. Furthermore, 35S-methionine pulsechase experiments showed that this protein is not produced from a preexisting one, even if protein synthesis is not inhibited (Krueger and Orme-Johnson 1983).