Meiotic recombination analysis in female ducks (Anas platyrhynchos)

PIGOZZI, MARÍA INÉS; DEL PRIORE, LUCÍA

GENETICA

SPRINGER

Lugar: Berlin; Año: 2016 vol. 144 p. 307 - 312

0016-6707

Meiotic recombination in female ducks wasdirectly studied by immunolocalization of MLH1 protein, amismatch repair protein of mature recombination nodules.In total, 6820 crossovers were scored along the autosomalsynaptonemal complexes in 122 meiotic nuclei. From thisanalysis we predict that the female map length of the duckis 2845 cM, with a genome wide recombination rate of2 cM/Mb. MLH1-focus mapping along the six largestbivalents shows regional variations of recombination frequenciesthat can be linked to differences in chromosomemorphology. From this MLH1 mapping it can be inferredthat distally located markers will appear more separated ingenetic maps than physically equidistant markers locatednear the centromeres on bivalents 1 and 2. Instead, markersat interstitial positions on the acrocentric bivalents 3?6 willappear more tightly linked than expected on the basis oftheir physical distance because recombination is comparativelylower at the mid region of these chromosomes. Thepresent results provide useful information to complementlinkage mapping in ducks and extend previous knowledgeabout the variation of recombination rates among domesticGalloanserae.