Reactive oxygen species mediate dopamine-induced signaling in renal proximal tubule cells

ACQUIER, A; MORI SEQUEIROS GARCIA, MM; GOROSTIZAGA, AB; PAZ, C; MENDEZ, CF

FEBS LETTERS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2013 vol. 587 p. 3254 - 3260

0014-5793

Intrarenally-produced dopamine (DA) induces a large increase in urinary sodium excretion mainly due to the inhibition of tubular sodium reabsorption. We aimed to study the participation of reactive oxygen species (ROS) in DA signaling pathway in proximal tubule cells. Our results show that DA increased ROS production in OK cells and indicate the mitochondria as the main source of ROS. DA also increased ERK1/2, superoxide dismutase (SOD) and transcription factor kB (NF-kB) activity. These findings suggest that DA generates mitochondria-derived ROS that activate ERK1/2 and subsequently NF-kB and SOD activity at concentrations that exert a physiological regulation of renal function.