INBIOMED   24026
INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Unidad Ejecutora - UE
artículos
Título:
Idebenone treatment mediates the effect of menadione oxidative stress damage in Saccharomyces cerevisiae.
Autor/es:
OLIVER GAMONDI, SEBASTIÁN CHAPELA, INÉS NIEVAS, ISABEL BURGOS, MANUEL ALONSO AND CARLOS STELLA
Revista:
Drug Metabolism Letters
Editorial:
Bentham Science Publishers
Referencias:
Año: 2012
ISSN:
1872-3128
Resumen:
We investigated the damage caused by oxidative stress using the yeast Saccharomyces cerevisiae as a model biological system. After inducing oxidative stress with menadione, we were able to evaluate the extent of cellular oxidative stress by utilizing 2´,7´-dichlorofluorescein diacetate (DCFH-DA) as a marker marker of the presence of reactive oxygen species. Cells were grown on different carbon sources in order to compare fermentative and oxidative metabolism. Under these conditions we evaluated the effectiveness of idebenone (2,3-dimethoxy-5-methyl-6-(10-hydroxydecyl)-1,4-benzoquinone) as a molecule that could relieve menadione-induced growth inhibition in Saccharomyces cerevisiae.