Role of PGE2 on neutrophils during human tuberculosis.

PELLEGRINI JM; AMIANO N.O.; GARCIA V.E.; MORELLI MP; CIALLELLA L.; MORELLI MP; CIALLELLA L.; MARTIN C.; TATEOSIAN N.L.; PALMERO D.J.; MARTIN C.; TATEOSIAN N.L.; PALMERO D.J.; PELLEGRINI JM; AMIANO N.O.; GARCIA V.E.

Simposio; Keystone eSymposia. Tuberculosis: Science Aimed at Ending the Epidemic.; 2020

Tuberculosis (TB) is one of the top 10 causes of death worldwide. Prostaglandin E2 (PGE2), an active lipidcompound derived from arachidonic acid, regulates different stages of the response of the host during severalpathologies such as chronic infections or cancer. Interestingly, manipulation of PGE2 levels was proposed as anapproach for countering the Type I IFN signature of TB 1, although very limited information about this pathwayin tuberculosis patients is available. Therefore, the aim of the present work was to investigate the role of thiscompound during human TB. Neutrophils were obtained from heparinized peripheral blood from healthydonors (HD) and tuberculosis patients (TB) by Ficoll-Hypaque centrifugation and Dextran sedimentation. Cellswere cultured (2x106 cells/ml) with a Mycobacterium tuberculosis lysate (Mtb-Ag, 10μg/ml) with/withoutPGE2. Then, we evaluated the role of this lipidic mediator during the human immune response against Mtb-Agby using flow cytometry and confocal microscopy assays. P-values