Endoplasmic Reticulum Stress through ATF6a pathway is associated with an inflammatory response in endometrial stromal cells: potential regulation by miRNAs

E.SOCZEWSKI, J. MURRIETA-COXCA, L. MIRANDA , R.GUTIÉRREZ-SAMUDIO, L. GALLINO , E. GRASSO, L. FERNÁNDEZ, S. GORI, M.MARTI, D.MORALES-PRIETO, R. FAVARO, C. PÉREZ LEIRÓS, U.MARKERT, R. RAMHORST

Congreso; Reunión de anual de SCHCF y ALACF; 2020

During decidualization, endometrial stromal cells undergo endoplasmic reticulum stress (RS) and unfolded protein response (UPR), which will allow them to expand their endoplasmic reticulum with the corresponding machinery for protein folding. These processes are directed by miRNAs that regulate the expression or stability of their transcription factors. Here we focus on the role of RS and UPR during the decidualization to induce a physiological sterile inflammatory response and whether might be regulate by miRNAs. We used an in vitro model of decidualization represented by human telomerase-immortalized endometrial stromal cell line St-T1b, treated with 8-Br-cAMP (0.5 mM) during 5 days, or Thapsigargin (Tg, a RS-inducer 1 µg/ml); and endometrial biopsies from patients with recurrent spontaneous abortions (RSA) and recurrent in vitro fertilization failures (RIF).We evaluated the expression of the RS-sensor ATF6 and the UPR marker, CHOP. Both markers increased in decidualized cells, and Tg induce even higher levels in comparison with non-decidualized cells (p