Microencapsulation of probiotic lactic acid bacteria into a matrix of Argentine chitosan. Its application as extracellular vesicles-controlled delivery device and its impact in the host digestive tract

D´ANTONI, CL; PÉREZ, OE; PIURI, M; DOMINGUEZA RUBIO, AP

Congreso; 1st zooming into preclinical nanomedicines in the era of Covid; 2020

Asociación Argentina de Nanomedicinas (NANOMED-AR)

The aim of this work plan is to encapsulate the probiotic Lacticaseibacillus casei BL23 into anArgentine chitosan (Q) matrix in order to increase its survival during food processing, storage andthe transit through the digestive system, until reaching the gut, where it would function asextracellular vesicles (EVs)-delivery device. This strategy would protect bacteria from changes inpH, temperature and osmotic stress, as well as from bacteriophage attack during fermentationprocesses of food.The probiotic effect of L. casei BL23 EVs will be evaluated in in vitro, ex vivo and in vivo models ofinflammation. We hypothesize that microencapsulation would increase the residence time of thebacteria in the gut and thus its probiotic effects. In addition, the mucoadhesive properties of Qwould allow a sustained release of EVs. Bacterial EVs were able to pass through gut epithelialcells and reach the host bloodstream and finally its target tissues as demonstrated by Domínguez-Rubio et al 2020.Analytical techniques such as dynamic light scattering, nanoparticle tracking analysis, Z potentialdeterminations, electron, atomic force, fluorescence and confocal microscopy, molecular biologyand immunological techniques (ELISA, flow cytometry) will be used. Dehydration in spray systemswill be implemented to achieve microencapsulation.