VIP DEFICIENCY PROMOTES AN INFLAMMATORY OVARIAN MICROENVIRONMENT IMPAIRING PREGNANCY

ESTEBAN GRASSO; M. MAY; JAMES WASCHEK; VANESA HAUK; FERNANDA PARBORELL; ZAIRA NAGUILA; ROSANNA RAMHORST; LUCILA GALLINO; NATALIA PASCUALI; VANINA FONTANA; CLAUDIA PEREZ LEIROS

Congreso; Congreso de la Sociedad Argentina de Inmunología 2020; 2020

Background: Complex immune regulation is required to ensure asuccessful pregnancy outcome. Vasoactive intestinal peptide (VIP)is an immunoregulatory peptide known to induce a tolerogenic ma-ternal response. Moreover, VIP regulates folliculogenesis and ovu-lation as well as ovarian steroidogenesis. VIP KO mice are known tobe subfertile, so we aimed to better understand how VIP deficiencyaffects ovarian physiology and immune homeostasis.Methods: Animals: VIP Knockout KO (-/-), deficient HT (+/-) and wildtype WT (+/+) mice in estrus (defined by vaginal lavage observation)were studied at 3 or 8 months of age. Serum hormones were mea-sured with immulite Xpi Siemens platform. Ovaries were examinedafter histological staining, hormones quantified using RIA and IL-1secretion by ELISA.Results: Young KO mice showed cycling disorders accompanied byan altered P4/E2 ratio in serum and ovary in the day of estrus ascompared to WT mice of the same age. We also found histologicaldifferences between the ovaries, with an increase in secondary fol-licles, less corpora lutea, and more atretic follicles (p