Oral health and adverse pregnancy outcome: LPS from Porphyromonas gingivalis impairs trophoblast-neutrophil-regulatory T cell interaction.

V. HAUK, G. CALO, B. LARA, D. VOTA, L. GALLINO, F. MERECH, D. PAPARINI, R. RAMHORST, C. PEREZ LEIROS.

Mar del Plata

Congreso; Reunión Anual Conjunta SAIC-SAFE-SAB-SAP; 2019

SAIC-SAFE-SAB-SAP

Trophoblast cell differentiation into an invasive phenotype is crucial for an adequate placentation as well as trophoblast-immune cell interaction to maintain immune homeostasis. Porphyromonas gingivalis (Pg) is an important pathogen of periodontal disease that has been implicated in different adverse pregnancy outcome although the mechanisms involved are still unclear. The aim of this study was to examine the effect of Pg-LPS on trophoblast cell function and trophoblast-neutrophil interaction.Human trophoblastic cell line Swan-71 was treated with Pg-LPS (10ng/ml). Cytokine expression were evaluated by RTqPCR and ELISA, glucose uptake by flow cytometry using the fluorescent analogue 2-NBDG and cell invasion assessed in Matrigel-covered transwells. Peripheral blood neutrophils (Neu) were purified from healthy donors and cultured with conditioned media from trophoblast cells (TbCM) treated or not with LPS (PgLPS-CM); CD11b and reactive oxygen species (ROS) were evaluated by flow cytometry. Regulatory T cell induction was evaluated by flow cytomerty.Pg-LPS treatment reduces trophoblast cell invasion, glucose uptake and alteres cytokine production (IL1β secretion X±SE basal 11,4±4,3; Pg-LPS 21,36±5,4: P