VIP contribution to sustain immune tolerance during peri-implantation period: focus on Tregs recruitment

GRASSO ESTEBAN; BERENSZTEIN ESPERANZA; WASCHEK JAMES; HAUK VANESA; SARACO NORA; PARBOREL FERNANDA; RAMHORST ROSANNA; GALLINO LUCILA; VOTA DAIANA; PASCUALINI NATALIA; PEREZ LEIROS CLAUDIA

Congreso; LXIII Reunion anual Sociedad Argentina de Investigación clínica (SAIC), LXVI Reunion anual Sociedad Argentina de Inmunología (SAI), Reunión Anual de la Sociedad Argentina de Fisiología (SAFIS); 2018

Uterine receptivity and embryo implantation are two main processes that need a finely regulated balance between pro-inflammatory and tolerogenic mediators in order to allow a successful pregnancy. The immunopeptide VIP is a key regulator and is involved in the recruitment of regulatory T cells (Tregs) which are crucial in both periods. VIP-knockout mice show cycling disorders and pregnancy complications. Here, we analyzed, using two mice models, the relevance of VIP associated with the recruitment of Tregs. During the day of estrous we found histological differences between the uterus of WT mice vs VIP knockout mice (-/-) accompanied with undetectable expression levels of Foxp3, higher expression of RORγt and a decrease in IL-10 and VEGF detected by RT-PCR (p