IMIQUIMOD-INDUCED INHIBITION OF ANTIOXIDANT ENZYMES AND REACTIVE OXYGEN SPECIES ACCUMULATION IN MURINE HEMANGIOMA CELLS.

ROSA WAINSTOK; HERNÁN REINGRUBER; SILVINA GAZZANIGA; RODRIGO ROCCO; ADRIANA COCHON

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Infantile hemangiomas (IH) are the most common benign tumours of infancy, however intervention may be required when major complications are developed. Imiquimod (IQ), a TLR7/8 agonist, is a therapeutic alternative and previous in vitro studies of our laboratory have shown a cytotoxic selective effect of IQ towards hemangioma cells in terms of viability, migration and apoptosis triggering. The aim of this study was to investigate the ability of IQ to trigger reactive oxygen species (ROS) generation and its influence on antioxidant enzymes, prior to apoptosis of murine hemangioma cells. H5V cell line was treated with IQ (0, 5, 10 and 50 g/mL) for 2, 4 or 12 hours and analyzed for ROS generation and mitochondrial stability by flow cytometry using fluorescent probes DCF-DA and MitoTracker Red CMXRos, respectively. Antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD) activities were assessed through disappearance of H2O2 (μmol H2O2/min*mg protein) and inhibition of the epinephrine oxidation (USOD/mg protein) respectively. Early after treatment with IQ (2 hs) there was a steep drop (7012%; p