PROTEOMIC CHARACTERIZATION OF THE SECRETOME FROM PROSTATE CANCER AND BONE PROGENITOR CELL CO-CULTURE

ANSELMINO NICOLAS; COTIGNOLA JAVIER; GERALDINE GUERON; LAGE VICKERS, SOFIA; VAZQUEZ, ELBA ; ALEJANDRA PAEZ; VALACCO PIA

Orlando

Congreso; American Association for Cancer Research, Prostate cancer: Advances in basic, translational and clinical research.; 2017

AACR

Prostate cancer (PCa) is one of the most frequent sites of primary tumor development in human beings. PCa tumor cells tend to disseminate to and form metastatic lesions in the bone. Given the evident prevalence and clinical significance of bone metastases, unraveling the mechanisms exploited by cancer to form these destructive lesions is of critical interest. This is partly due to the fact that the metastatic cascade encompasses many rate limiting steps demanding tumor cells to employ new mechanisms at each stage.Our work aimed at evaluating which soluble factors could mediate the chemical cross-talk between PCa cells and bone progenitor cells. For this purpose, we used co-culture transwell systems of PC3 (human PCa cells) with the pre-osteoclastic Raw264.7 or pre-osteoblastic MC3T3 cell lines (murine cells), where cells shared the culture media (CMs) without physical contact for 24h.We employed mass spectrometry -nanoLC-MS/MS(Orbitrap)- for the secretome analysis of the CMs. The obtained spectra were analyzed with the Proteome Discover Software and compared with both, human and murine protein databases. Results highlight a differential secretome profile released to the CM when tumor cells are grown in co-culture compared to controls. These results could potentially explain the altered mRNA expression levels assessed by RTqPCR in PCa cells growing in co-culture, displaying significant up-regulation for HO-1 ANXA2, ANXA2R,OPG and PTHrP (p