THE PRESENCE OF HOMOCYSTEINE AND ADENOSINE EXACERBATED THE TNF- α CYTOTOXICITY IN HUMAN ERYTHROLEUKEMIA CELLS

MALTANERI, R.; VITTORI, D.; SCHIAPPACASSE, A.; NESSE, ALCIRA B.; WETZLER, D.

Ciudad Autonoma de Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias. LXII Reunión Anual de la Sociedad Argentina de Investigaciones Clínicas; 2017

Sociedad Argentina de Investigación Clínica (SAIC)

Some factors are known to influence cell survival regulation, includingTNF-α and cellular hypomethylation, which can be induced by high levels ofhomocysteine and adenosine. S-adenosylhomocysteine (SHA) production isaccelerated by the reversible enzyme SAH hydrolase during the methioninemetabolism. Both, hyperhomocysteinemia (hHcy) and elevated SAH has beendescribed in end stage renal disease (ESRD) and associated with severalcellular abnormalities in these patients. Other negative prognosis factors inESRD are inflammatory conditions leading to anemia. The aim of this study wasto investigate if hHcy and the accumulation of adenosine could affect thesurvival of human erythroleukemia K562 cells in an inflammatory environment andwhether the growth factor erythropoietin (Epo) could reverse this effect.Cells were incubated (2 h) with Hcy (H 500 µM) and adenosine (A 250 µM)in free serum medium and then exposed (24 h) to TNF-α (T 60 ng/mL). Cellviability measured by the MTT assay and expressed as OD was: C 1.10±0.17; *HAT0.43±0.18; *P