Oxidative stress and altered Mitochondrial Quality Control in blue light-mediated damage of A2E-containing Retinal Pigment Epithelium cells

KOTLER ML; GOROJOD RM.; GARCIA LIÑARES G; GRECCO HE; PORTE ALCON S; ALAIMO A; BALDESSARI A; BUJJAMER JM

Paris

Congreso; 20th International Conference on Oxidative Stress Reduction, Redox Homeostasis and Antioxidants; 2018

International Society of Antioxidants - Université Pierre and Marie Curie (UPMC)

Age-related macular degeneration (AMD) is a neurodegenerative retinal disease. Accumulated lipofuscin fluorophore A2E in retinal pigment epithelium (RPE) confers susceptibility to blue light-mediated damage, an AMD risk factor. RPE dysfunction is a critical event in AMD. Aim: evaluate mitochondrial quality control under photooxidative damage conditions in RPE. Light-emitting diodes irradiation (λ=445nm; 1.7mW/cm2, 30min) significantly reduced the viability of both non-pigmented and 10 or 25μM A2E-containing human RPE cells (MTT assay: 52%, 46%, 31%, respectively) as well as increased mitochondrial anion superoxide production (MitoSOX Red). 25µM A2E, along with blue light, induced apoptosis measured by Annexin V/PI-flow cytometry (6-fold, p