Mix of glyphosate and cypermethrin formulations induce oxidative stress in HEp2 cell line

COALOVA, ISIS; CHAUFAN GABRIELA; MARCH H; RIOS DE MOLINA M.C.

buenos aires

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

During the last few years, in Argentina there has been a strong process of expansion of glyphosate-resistant soybean crops. Along with this exponential growth, the use of agrochemicals has increased. It has been reported that many pesticides (including herbicides) generate intracellular reactive oxygen species (ROS). However, there is still much to be done to understand the underlying mechanisms in the case of pesticides mixtures. In the present study, we aimed to examine the effects on oxidative balance of the glyphosate and cypermethrin mixture (MIX) in HEp2 cell line compared to the effects of the separately formulations. For this purpose, we determined ROS formation, glutathione (GSH) equivalents content, antioxidant and detoxificant enzymes activity (superoxide dismutase (SOD), catalase (CAT), and GSH-S-transferase (GST) respectively), and carbonyl content as a marker of oxidative damage. Our results show that MIX exposure at NOAEL (no observed adverse effect level) produced a significant increase (p