S-layer proteins of Lactobacillus sp. as potential treatment for bacterial and virus pathogens

PRADO ACOSTA, MARIANO; GARCÍA, GÉNESIS; BARQUERO, ANDREA A.; RUZAL, SANDRA

Córdoba

Congreso; LII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica (SAIB); 2016

SAIB

Surface layer (S-layer) protein constitute in some species of lactobacilli their outermost cell envelope and have been considered to play a role in the probiotic properties of the strains. Here, we investigated the antibacterial and antiviral ability of S-layer proteins purified from Lactobacillus acidophilus ATCC4356, Lactobacillus brevis ATCC14869, Lactobacillus helveticus ATCC12046 and Lactobacillus kefiri JCM5818. Capacity to protect A549 cells (human lung adenocarcinoma cell line) against Pseudomonas aeruginosa adhesion and HSV-1 multiplication was evaluated. We found that S-layers alter the Pseudomonas pattern of adhesion to A549 cells, from a diffuse adhesion in control cells to a localized or aggregative form in pretreated cells. These results were accompanied by a reduction in the number of bacterial adherence, as evidenced by flow cytometry and plate count. Also, we examined their effect on Ps. aeruginosa biofilm formation. Likewise, the standard crystal violet staining procedure shown that these proteins impede biofilm formation without causing bactericidal effects on planktonic bacteria. The antiviral assay revealed that S-layers of L. acidophilus, L. brevis and L. helveticus have an interesting antiviral effect against HSV-1 at subtoxic concentrations. The results provide knowledge of important application of S-layer as treatment for bacterial and virus pathogens.