Genomic study of mitochondrial DNA and nuclear genes coding for mitochondrial proteins in prostate cancer

OLIVIERI, CONRADO; COTIGNOLA, JAVIER; GUERON, GERALDINE; CANTOR, FEDERICO; VAZQUEZ, ELBA

Mar del Plata

Congreso; LXI Reunion Anual de la Sociedad Argentina de Investigacion Clinica; 2016

Sociedad Argentina de Investigacion Clinica

Prostate cancer (PCa) is the second most frequently type of cancer and the sixth major cause of cancer-related mortality in men. The predictive clinical parameters include the Gleason grade, the tumoral extensions and the precirurgical PSA. There are several studies about how the mtDNA mutations and deletions and mitochondrial function alterations are early indicators of malignant prostatic transformation. Our objectives are to study the mitochondrial role in PCa at genomic level, using public clinical data from RNAseq (n=71) and expression microarrays (primary tumor n=144, normal tissue adjacent to tumor n=60, metastatic patients n=22 and healthy patients n=17) of PCa patients with clinical information (Gleason grade, PSA, T stage and relapse) and find molecular markers that allow us to improve the specificity of clinical parameters. For this task, we used bioinformatics programs like, ?Bowtie?, ?Tophat? and ?Cufflinks? to realize RNAseq?s reads alignments. Also we used ?IGV? for searching for SNP?s and deletions. We found that samples with G. grade >7 presents mutations in the mitochondrial gene COX2, while samples with G. grade