IQUIBICEN 23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Immunoregulation of the decidualization program
Autor/es:
IMMUNOREGULATION OF THE DECIDUALIZATION PROGRAM. L. GALLINO, E. GRASSO, E. SOCZEWSKI, S. GORI, G. MARTINEZ, G. SALAMONE, C. PEREZ LEIRÓS, R. RAMHORST.
Lugar:
Buenos Aires
Reunión:
Congreso; LASID-FAIC-FAIC Meeting,; 2015
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
Decidualization process involves phenotype and functional changes on endometrial cells and the modulation of different mediators such as cytokines, chemokines and the selective recruitment of immune cells. The vasoactive intestinal peptide (VIP) is a neuropeptide produced by endometrial stromal cells involved in immunetolerance.Human endometrial stromal cell line (HESC) was treated or not with VIP or with medroxiprogesterone (MPA) and dbcAMP. VIP/VPAC system and decidualization markers were evaluated by RTPCR and ELISA. Invasion assay was performed using blastocyst-like spheroids (BLS) from first trimester trophoblast cells (Swan71) cultured on HESC monolayer. The activation profile of dendritic cells (DC) and monocytes, and the migration of monocytes and Treg cells were evaluated by flow cytometry. Foxp3 expression was evaluated on C57BL/6 wildtype or VIP-deficient mice uterus.Decidualization increased VIP expression and secretion on HESC cells (p
