THE IL-17 RS2275913 SINGLE NUCLEOTIDE POLYMORPHISM (SNP) IS ASSOCIATED WITH HUMAN TUBERCULOSIS SUSCEPTIBILITY IN ARGENTINA.

ROLANDELLI, A.; HERNANDEZ DEL PINO, R.E.; ÁLVAREZ, G.I.; PELLEGRINI, J.M.; TATEOSIAN, N.L.; AMIANO, N.O.; PALMERO, D.J.; CASCO, N.; GUTIERREZ, M.; PASQUINELLI, V.; GARCÍA, V.E.

Capital Federal

Congreso; I Meeting LASID FAIC SAI; 2015

Abstract: Background. Interleukin 17A (IL-17) plays an important role in host-pathogen interaction during Mycobacterium tuberculosis (Mtb) infection. Genetic variations in IL-17 gene may affect the immune response against this bacterium. The SNP rs2275913 (g/a) is the most studied nucleotide substitution in IL-17A gene, but only one study reported the association of this SNP with tuberculosis susceptibility in a Spanish population. Here we investigated the relationship between rs2275913 SNP and tuberculosis susceptibility in Argentina and evaluated the functional implications of the substitution.Methods. 146 tuberculosis patients (TB) and 164 healthy donors (HD) were genotyped by ARMS-PCR. IFN-gamma and IL-17A levels were analyzed by ELISA in plasma and supernatants from peripheral blood mononuclear cells (PBMCs) stimulated with a Mtb lysate.Results. Significant differences in the allelic and genotypic (p