The IL-17 RS2275913 single nucleotide polymorphism (SNP) is associated with human tuberculosis susceptibility in Argentina

ROLANDELLI AGUSTIN; HERNANDEZ DEL PINO RODRIGO; ALVAREZ GUADALUPE I; PELLEGRINI JOAQUIN M; TATEOSIAN NANCY L; AMIANO NICOLAS O; PALMERO DOMINGO J; GUTIERREZ MARISA; PASQUINELLI VIRGINIA; GARCÍA VERÓNICA E

Congreso; IV Congreso de la Sociedad Latinoamericana de Inmunodeficiencias (LASID), LXIII Reunión de la Sociedad Argentina de Inmunología (SAI) y II Reunión FAIC (French-Argentinean Immunology Congress); 2015

Background. Interleukin 17A (IL-17) plays an importantrole in host-pathogen interaction during Mycobacteriumtuberculosis (Mtb) infection. Genetic variations in IL-17 gene mayaffect the immune response against this bacterium. The SNPrs2275913 (g/a) is the most studied nucleotide substitution in IL-17A gene, but only one study reported the association of this SNPwith tuberculosis susceptibility in a Spanish population. Here weinvestigated the relationship between rs2275913 SNP andtuberculosis susceptibility in Argentina and evaluated thefunctional implications of the substitution.Methods. 146 tuberculosis patients (TB) and 164 healthy donors(HD) were genotyped by ARMS-PCR. IFN-gamma and IL-17Alevels were analyzed by ELISA in plasma and supernatants fromperipheral blood mononuclear cells (PBMCs) stimulated with aMtb lysate.Results. Significant differences in the allelic and genotypic(p