Alterations in endometrial programming leads to recurrent in vitro fertilization failures

. L CASTAGNOLA, M SGORI, E SOCZEWSKI, E GRASSO, L FERNÁNDEZ, A SCHAFIR, M IRIGOYEN, G MARTÍNEZ, L GALLINO, C PÉREZ LEIRÓS Y R RAMHORST.

Mar del plata

Congreso; Reunión Anual Conjunta SAIC-SAI-SAFIS; 2022

Objectives: During the decidualization process, a subset of endometrial stromal cells (EnSCs) undergoes cellular senescence. We hypothesize that recurrent implantation failure (RIF) patients display an imbalance between both subpopulations. Since these patients have been reported to show alterations in endoplasmic reticulum stress (ERS), we studied the link between both senescence and ERS using an in vitro model.Materials and methods: Deiodinase 2 (DIO2), Lumican (LUM) and Clusterin (CLU) were evaluated as markers for the senescent subset, while Ferritin (FTL) was used to evaluate the decidual one. Forkhead box protein O1 (FOXO1) was tested as a key transcription factor involved in the divergence of the subpopulations. Gene expression was tested in endometrial biopsies from fertile women, RIF patients and Human Endometrial Stromal Cell line (HESC) by RT-qPCR. To evaluate how ERS affects senescence in EnSCs, HESC cells were ERS induced using 1ug/ml of thapsigargin (Tg) for 4h. Then, stimulus was washed, and supernatants and RNA were recovered after 48 h *p