Manganese-exposed BV-2 microglia induces damage in N27 dopaminergic neuronal cells.

VINUESA, ANGELES; GOROJOD, ROXANA MAYRA; ABIUSO, ADRIANA MARÍA BELÉN; SARAVIA, FLAVIA; PORTE ALCON, SOLEDAD; KOTLER, MÓNICA LIDIA

Virtual

Congreso; Reunión conjunta de Sociedades de Biociencias; 2021

Sociedad Argentina de Investigación Clínica, Sociedad Argentina de Inmunología, Asociación Argentina de Nanomedicinas, Asociación Argentina de Farmacología Experimental

Manganese (Mn) intake is essential at physiological concentrations. However, prolonged exposure produces a neurodegenerative disease called manganism, whose symptoms are often confused with idiopathic Parkinson´s disease. Even though the harmful effect of Mn in different cell types has been described, much remains to be understood regarding the outcomes of glial activation on neuronal death. Objective: To evaluate the effect of soluble mediators released by microglial cells after Mn exposure on neuronal integrity. For this purpose, we first characterized the direct effect of Mn exposure on neuronal and microglial cells, and then explored the influence of microglia-conditioned medium (MCM) on neurons viability. Methodology: MCMs were generated by BV-2 cells incubation with 250-1000 µM Mn for periods of 3 or 6 h, and were used to stimulate N27 neuronal cells for 24h; cell viability was assessed using MTT reduction assay; the study of ROS production was performed using DCFDA; the change in mRNA expression was quantified by RT-qPCR. Results: In N27 neuronal cells, Mn exposure induced a concentration-dependent decrease in cell viability after 24h (100-1000 µM, P