COLLOIDAL SYSTEMS FOR ANTIVIRAL DRUGS ENCAPSULATION FOR NEGLECTED DISEASES

DODESTRAIAN MARTÍN M.; SEPULVEDA CLAUDIA S.; CARLUCCI MARÍA JOSEFINA; SÁNCHEZ DOMINGUEZ MARGARITA; CASTAÑEDA CATAÑA MA; PÉREZ, OSCAR EDGARDO

Seattle

Congreso; 34th International Conference on Antiviral Research; 2021

INTERNATIONAL SOCIETY FOR ANTIVIRAL RESEARCH

COLLOIDAL SYSTEMS FOR ANTIVIRAL DRUGS ENCAPSULATION FOR NEGLECTED DISEASESCastañeda Cataña, M. Alejandra1, 2; Dodes Traian, Martín M.1,2; CarlucciJosefina1,2 Pérez, Oscar E.1, 2; Sepúlveda, Claudia S.1, 2.; Sánchez Domínguez Margarita31 Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica, Buenos Aires, Argentina.2 CONICET-Universidad de Buenos Aires. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN). Buenos Aires, Argentina.3 Laboratorio de Química Coloidal e Interfacial, Centro de Investigación en Materiales Avanzados S.C. (CIMAV), Unidad MonterreyThe emergence of viral resistant mutants to antiviral drugs is the most important issue in current antiviral therapy, so an ideal therapeutic goal to prevent this is represented by host factors that are crucial to the viral replicative cycle. Poor pharmacokinetic profiles and resistance are the main disadvantages of the currently used antiviral agents, so they are excellent research objectives, especially in presence of viral pandemics such as DENV, ZIKV, HIV, Hepatitis. Mycophenolic acid (MPA) is a non-nucleoside and non-competitive inhibitor of inosine monophosphate dehydrogenase (IMPDH), clinically safe and usually used as an immunosuppressant for prevention of rejection of transplanted organs. To solve cytotoxicity problems, sustained drug delivery systems are required through the use of nanoparticles. Nanocarriers can provide key advantages for drugs or therapeutic molecules in vivo administration.We have confirmed the binding interaction between MPA and bovine seroalbumin protein (BSA) by fluorescence spectroscopy with a Kd = 12.0 ± 0.7 µM. We obtained BSA-nanoparticles of 59 ± 9 nm, PLGA-nanoparticles of 65 ± 10 nm and Niosomes of 85 ± 9 nm, determined by scanning electron microscopy (SEM). The BSA-MPA formulations did not show cytotoxicity in the range of concentrations tested; however, although they were active against Junín arenavirus, their antiviral effect was not superior to free MPA.