Antibody-independent and dependent infection of human myeloid cells with dengue virus is inhibited by carrageenan

QUINTANA V; CARRO AC; PICCINI LE; DAMONTE EB; QUINTANA V; CARRO AC; PICCINI LE; DAMONTE EB

VIRUS RESEARCH

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2020 vol. 290

0168-1702

This study demonstrated that the lamda-carrageenan is a potent and selective inhibitor of the primary infection of human myeloid U937 and K562 cells with the four DENV serotypes, achieving a higher than 99% reduction in virus production at the highest tested concentration of 20 µg/ml, without affecting cell viability at concentrations up to 1000 µg/ml. Since antibody-dependent enhancement (ADE) is thought to play a main role in the aggravation of severe DENV disease, we also evaluated the activity of carrageenan against ADE of DENV infection. Our results showed that the lamdba-carrageenan was also effective to block the antibody dependent infection of both cell lines mediated by Fcgamma-RII, a property non specific of DENV serotype. Mechanistic studies indicated that DENV-2 entry is the main antiviral target for carrageenan in DENV or DENV-Ab infected human myeloid cells. Then, carrageenan may be considered an interesting alternative among the few compounds known to be able to block DENV entry during both primary and antibody-dependent infection.