IQUIBICEN 23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
artículos
Título:
Exogenous human OKSM Factors Maintain Pluripotency Gene Expression of Bovine and Porcine iPS-like cells Obtained with STEMCCA Delivery System
Autor/es:
CANIZO J; ALLER ATUCHA J; ALBERIO R; KLISCH D; ALBERIO RH; VAZQUEZ ECHEGARAY C; PAZ D; GUBERMAN A
Revista:
BMC Research Notes
Editorial:
Springer link
Referencias:
Año: 2018
ISSN:
1756-0500
Resumen:
Objectives: The use ofinduced pluripotent stem (iPS) cells as an alternative to embryonic stem cells toproduce transgenic animals requires the development of a biotechnologicalplatform for their generation. In this study, different strategies for thegeneration of bovine and porcine iPS cells were evaluated. Lentiviral vectorswere used to deliver human factors OCT4, SOX2, KLF4 and c-MYC (OKSM) intobovine and porcine embryonic fibroblasts and different culture conditions wereevaluated. Results: Protocols based onthe integrative lentiviral vector STEMCCA produced porcine iPS-like cells moreefficiently than in bovine cells. The iPS-like cells generated displayed stemcell features; however, expression of exogenous factors was maintained along atleast 12 passages. Since inactivation of the exogenous factors is still a majorbottleneck for establishing fully reprogrammed iPS cells, defining cultureconditions that support endogenous OKSM expression is critical for the efficientgeneration of farm animals? iPS cells.
