IQUIBICEN 23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
artículos
Título:
Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria
Autor/es:
HATFULL, GRAHAM F.; PIURI, MARIANA
Revista:
METHODS IN MOLECULAR BIOLOGY (CLIFTON, N.J.)
Editorial:
Humana Press Inc.
Referencias:
Año: 2019 vol. 1898 p. 27 - 36
ISSN:
1064-3745
Resumen:
Fluoromycobacteriophages are a new class of reporter phages that contain Laboratorio fluorescent reporter genes (gfp, ZsYellow, and mCherry) and provide a simple means of revealing the metabolic state of mycobacterial cells and therefore their response to antibiotics. Here we described a simple and rapid method for drug susceptibility testing (DST) of Mycobacterium spp using a fluorescence microscope, a flow cytometer, or a fluorimeter in a convenient multiwell format.
