IQUIBICEN 23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
artículos
Título:
A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: Performance in vitro and in live cells
Autor/es:
TOUM TERRONES, YAMILI; COLUCCIO LESKOW, FEDERICO; BORDONI, ANDREA V.; ACEBEDO, SOFÍA L.; SPAGNUOLO, CARLA C.; WOLOSIUK, ALEJANDRO; TOUM TERRONES, YAMILI; COLUCCIO LESKOW, FEDERICO; BORDONI, ANDREA V.; ACEBEDO, SOFÍA L.; SPAGNUOLO, CARLA C.; WOLOSIUK, ALEJANDRO
Revista:
Journal of Materials Chemistry B
Editorial:
Royal Society of Chemistry
Referencias:
Año: 2017 vol. 5 p. 4031 - 4034
ISSN:
2050-7518
Resumen:
We report the synthesis of a near-infrared (NIR) fluorescent pH probe with a remarkable Stokes shift (∼135 nm) based on a tricarbocyanine (Cy-PIP). The fluorescent molecule was anchored to SiO2 nanoparticles (Cy-PIP@SiO2) and is capable of monitoring pH changes within the physiological range (pH 6-8). The Cy-PIP@SiO2 nanoparticles were successfully internalized by HeLa cells as shown by fluorescence confocal microscopy, while flow cytometry revealed pH fluctuations during the endocytic pathway.
