Genes of different catabolic pathways are coordinately regulated by Dal81 in Saccharomyces cerevisiae

PALAVECINO MARCOS; CORREA GARCIA SUSANA; BERMUDEZ MORETTI MARIANA

Journal of Amino Acids

Hindawi Publishing Corporation

Lugar: Cairo; Año: 2015 vol. 2015 p. 1 - 8

2090-0112

Yeast can use a wide variety of nitrogen compounds. However, the abilityto synthesize enzymes and permeases for catabolism of poor nitrogensources is limited in the presence of a rich one. This general mechanismof transcriptional control is called nitrogen catabolite repression. Poornitrogen sources, such as leucine, -aminobutyric acid (GABA) andallantoin, enable grow after the synthesis of pathway-specific catabolicenzymes and permeases. This synthesis occurs only under conditions ofnitrogen limitation and in the presence of a pathway-specific signal. Inthis work we studied the temporal order in the induction of AGP1, BAP2,UGA4 and DAL7, genes that are involved in the catabolism and use ofleucine, GABA and allantoin, three poor nitrogen sources. We found thatwhen these amino acids are available, cells will express AGP1 and BAP2 inthe first place, then DAL7 and at last UGA4. Dal81, a general positiveregulator of genes involved in nitrogen utilization related to themetabolisms of GABA, leucine and allantoin, plays a central role in thiscoordinated regulation.