Glyphosate commercial formulation cause cytoxicity, oxidative effects and apoptosis on human cells; differences with its active ingredient.

CHAUFAN G,; COALOVA I,; RÍOS DE MOLINA MC,

INTERNATIONAL JOURNAL OF TOXICOLOGY

SAGE PUBLICATIONS INC

Lugar: Thousand Oaks, California.; Año: 2014 vol. 33 p. 29 - 38

1091-5818

In the present study, the effects on oxidative balance and cellular endpoints of glyphosate, aminomethylphosphonic acid and a glyphosate-formulation were examined in HepG2 cell line, at dilutions levels far below agricultural recommendations. Our results show that glyphosate-formulation had toxic effects while no effects were found with acid glyphosate and aminomethylphosphonic acid treatments. Glyphosate-formulation exposure produced an increase in reactive oxygen species and nitrotyrosine formation, superoxide dismutase activity and glutathione levels, while no effects were observed for catalase and glutathione-S-transferase activities. Also, glyphosate-formulation triggered caspase 3/7 activation and hence induced apoptosis pathway in this cell line. Aminomethylphosphonic acid exposure produced an increase in glutathione levels while no differences were observed in others antioxidant parameters. No effects were observed when the cells were exposed to acid glyphosate. These results confirm that glyphosate-formulations have adjuvants working together with the active ingredient and causing toxic effects that are not seen with acid glyphosate.